Ventura County FC – Irvine Zeta FC

Colorado Rapids 2 – Northern Colorado Hailstorm FC

Union Omaha – Des Moines Menace

Chicago Fire FC II – Forward Madison FC

Apotheos FC – Chattanooga Red Wolves SC

Vermont Green FC – Carolina Core FC

One Knoxville SC – Greenville Triumph SC

South Georgia Tormenta FC – Savannah Clovers FC

Miami United FC – Club De Lyon

Minnesota United FC 2 – Michigan Stars FC